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Demethylation and auto-oxidation of different cobalt-bleomycin complexes
Summary
Type:
HPLC
Application #:
103680
Catalog#:
720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection:
UV, 254nm
Conditions:
eluent: 1% ammonium acetate - methanol (6:4, v/v)
flow rate 2ml/min
Authors:
Vos CM
Source:
J Antibiotics 35 (1982) 837 - 842
Details
Legend: Fig. . Chromatograms of Co-bleomycin-demethyl A2 complexes a) Co-blm A2, demethylated by heating at 120°C overnight b) 0.7mM blm dA2, chelated with cobalt, chromatographed immediately after preparat
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